Gastric cancer cells induce human CD4+Foxp3+ regulatory T cells through the production of TGF-β1

Figure 1 Gastric cancer cell supernatant induces the increased Tregs in coculture with peripheral blood mononuclear cells. A: Representative flow cytometry analysis of CD4⁺Foxp3⁺ Tregs frequency in CD4⁺ T cells population following peripheral blood mononuclear cells co-culture with medium, GES, or MGC supernatants. Rectangles show double positive gating and numbers reflect percentage of cells in that gate; B: Summarized data from all subjects showed that CD4⁺Foxp3⁺ Tregs increased in coculture with MGC supernatants (^aP < 0.05); C: Relative quantity of Foxp3 mRNA was measured by real-time polymerase chain reaction before and after culture with medium, GES, or MGC supernatants.