Tetracycline-inducible protein expression in pancreatic cancer cells: Effects of CapG overexpression

Figure 5 Two-dimensional gel analysis of stable inducible CapG overexpressing cells. A: Colloidal Coomassie Blue stained gels displaying the proteome of Sβtet29Cap35 (C35), Sβtet29Cap43 (C43) and control Sβtet29 (TET29) cells. Cells were lysed in their uninduced state (- dox) and 18 h after treatment with 500 ng/mL doxycycline (+ dox). The area around the spot representing CapG (black circle) was magnified in the right hand column (CapG); B: The spot representing CapG was excised from the gels, trypsin digested, and analysed by Maldi-Tof. The sequence is shown and peptides identified by Maldi are bold and underlined.