Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene

Figure 2 Restoration of miR-622 promotes gastric cancer cell invasion and migration. A: q-real time polymerase chain reaction for the expression of miR-622 and ING1 was performed using six gastric cancer cell lines. The lowest and highest levels of miR-622 expression among the six gastric cancer cell lines were found in SGC-7901 and NCI-N87 cells, respectively, and the expression of miR-622 was inversely correlated with the expression of ING1; B: Expression of miR-622 was restored or suppressed in gastric cancer cells after miR-622 precursor or miR-622 inhibitor transfection, compared with the controls (P < 0.01); C and D: Over-expression of miR-622 promotes increased cell invasion and migration compared with the miR-control transfectants. However, down-regulation of miR-622 inhibited cell invasion and migration compared with AS-control transfectants. ^bP < 0.01, compared with miR-control transfectants. Scale bars: 500 μm. AS-miR-622: An ambion miRNA inhibitor for miR-622.