Computational prediction and experimental validation of novel markers for detection of STEC O157:H7

doi:10.3748/wjg.v17.i14.1910

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 14, 2011; 17(14): 1910-1914
Published online Apr 14, 2011. doi: 10.3748/wjg.v17.i14.1910

Table 3 Candidate marker genes for Shiga toxin producing Escherichia coli O157:H7

EDL933	Sakai	EC4115	3000 bp flanking regions				Function
EDL933	Sakai	EC4115	Ident %	tRNA	Tpase	phage	Function
wzy	ECs2844	ECH74115_2973	78	-	-	-	O antigen polymerase
Z0372	ECs0334	ECH74115_0348	56	-	-	-	Hypothetical protein
Z0344	ECs0307	ECH74115_0324	50	-	-	-	Hypothetical protein
Z1539	ECs1281	ECH74115_1278	50	-	-	-	Hypothetical protein
Z3621	ECs3239	ECH74115_3589	13	-	-	-	Hypothetical protein
Z3271	ECs2909	ECH74115_3086	0	-	-	-	Hypothetical protein
Z0948	ECs0804	ECH74115_0880	9	-	-	+	Hypothetical protein
Z1328	ECs1061	ECH74115_1143	32	-	-	+	Hypothetical protein
Z1430	ECs1165	ECH74115_3572	0	-	-	+	Hypothetical protein
Z3348	ECs2979	ECH74115_3543	0	+	-	+	Hypothetical protein
Z3118	ECs2755	ECH74115_2802	0	-	-	+	Hypothetical protein
Z0244	ECs0212	ECH74115_0230	38	-	+	+	Hypothetical protein
Z1153/Z1592	ECs5413	ECH74115_1331	50	-	-	-	Hypothetical protein
Z2107/Z2378/ Z6055/Z3108	ECs1960/ ECs2748	ECH74115_2190/ ECH74115_2260/ ECH74115_2792	0	+	+	+	Hypothetical protein
Z1782/Z6064	ECs2271	ECH74115_3158/ ECH74115_1841/ ECH74115_2270/ ECH74115_1520	2	-	-	+	Hypothetical protein

“Ident %” is the identity percentage of 3000-bp flanking regions among the three genomes. “+” and “-“ in column “tRNA”, “Tpase” and “phage” represent if the 3000-bp flanking regions contain any tRNA, transposase or phage. The gene names and their functions were retrieved from the NCBI Prokaryotic Genome Database, as described in materials and methods.

Citation: Wang GQ, Zhou FF, Olman V, Su YY, Xu Y, Li F. Computational prediction and experimental validation of novel markers for detection of STEC O157:H7. World J Gastroenterol 2011; 17(14): 1910-1914
URL: https://www.wjgnet.com/1007-9327/full/v17/i14/1910.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i14.1910