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©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 14, 2011; 17(14): 1910-1914
Published online Apr 14, 2011. doi: 10.3748/wjg.v17.i14.1910
Published online Apr 14, 2011. doi: 10.3748/wjg.v17.i14.1910
Table 2 Oligonucleotide primer sequences and parameters used for detection Shiga toxin producing Escherichia coli O157:H7
| Gene | sequence (5' to 3' ) | Expected | PCR condition |
| length (bp) | Ta/ Te (°C) | ||
| eaeA | F-AAGCGACTGAGGTCACT | 45 | 55/60 |
| R-ACGCTGCTCACTAGATGT | |||
| wzy | F-GAACGATTTCTTTCCGACACC | 276 | 50/60 |
| R-GCGCAATTTATCGAGCTATG | |||
| Z037 | F-AGAATCTCATCCTCGCATTT | 342 | 52/60 |
| R-TCTCGCAGTTTCGCATCTTAT | |||
| Z0344 | F-ATTGTCAGGGAAATTAGCGTG | 121 | 51/60 |
| R-TGCTGTTAATGGTTGAACCGA |
- Citation: Wang GQ, Zhou FF, Olman V, Su YY, Xu Y, Li F. Computational prediction and experimental validation of novel markers for detection of STEC O157:H7. World J Gastroenterol 2011; 17(14): 1910-1914
- URL: https://www.wjgnet.com/1007-9327/full/v17/i14/1910.htm
- DOI: https://dx.doi.org/10.3748/wjg.v17.i14.1910