Differential expression of Bcl-2 and Bax during gastric ischemia-reperfusion of rats

Figure 1 Histological exhibition of Bcl-2 and Bax positive cells in the gastric mucosa at different reperfusion times after ischemia, by immunohistochemical staining in rats. The Bcl-2 and Bax positive cells were respectively probed with anti-Bcl-2 and anti-Bax polyclonal antibodies in rat gastric mucosa. Nuclear counterstaining was performed with hematoxylin. The examples of immunoreactive cells are those with dark brown staining in their cytosol (arrows). A and B: Bcl-2, control; C: Bcl-2, GI-R at 1 h after reperfusion; D: Bcl-2, GI-R at 24 h after reperfusion; E and F: Bax, control; G: Bax, GI-R at 1 h after reperfusion; H: Bax, GI-R at 24 h after reperfusion. Images were obtained at × 100 (A, C, D, E, G and H, Bar 100 μm) and × 400 (B and F, Bar 400 μm).