Legalon-SIL downregulates HCV core and NS5A in human hepatocytes expressing full-length HCV

Figure 4 Effects of legalon-SIL on heme oxygenase-1, Bach1, and nuclear factor erythroid 2-related factor 2 mRNA levels after 24 h. CON1 cells were grown to near confluence and medium was changed to 5% FBS plus Dulbecco’s modified Eagle’s medium, then treated with vehicle only (DMSO) or 50 or 200 μmol/L legalon-SIL (LS). The cells were harvested 24 h after treatment. The levels of HMOX-1, Bach1, and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA were quantified using quantitative reverse transcriptase polymerase chain reaction as described in “Materials and Methods”. The amounts of HMOX-1, Bach1, and Nrf2 mRNA were normalized to glyceraldehyde-3-phosphate dehydrogenase. Data are presented as the mean ± SE experiments were performed 3 times. ^bP < 0.01 vs DMSO control.