Legalon-SIL downregulates HCV core and NS5A in human hepatocytes expressing full-length HCV

Figure 3 Effects of legalon-SIL on hepatitis C virus core and NS5A protein levels in CON1 cells after 24 and 48 h. CON1 cells were grown to near confluence and the medium was changed to 5% FBS plus Dulbecco’s modified Eagle’s medium, then treated with vehicle only (DMSO) or 50 or 200 μmol/L legalon-SIL (LS). The cells were harvested 24-48 h after treatment. Total protein (10 μg) was separated on 4%-12% gradient sodium dodecyl sulphate-polyacrylamide gel electrophoresis and electrophoretically transferred onto an Immun-Blot PVDF. Hepatitis C virus core and NS5A protein levels were assessed through Western blotting. Representative Western blots, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-normalized quantifications are shown after 24 h (A, B), and 48 h (C, D). Data for protein levels are mean ± SE (n = 3 independent experiments). ^aP < 0.05, ^bP < 0.01 vs DMSO control.