Legalon-SIL downregulates HCV core and NS5A in human hepatocytes expressing full-length HCV

Figure 2 Time course of effects of legalon-SIL on hepatitis C virus RNA in CON1 cells. CON1 cells were grown to near confluence and the medium was changed to 5% fetal bovine serum (FBS) plus Dulbecco’s modified Eagle’s medium, then treated with vehicle only (DMSO) or 50 or 200 μmol/L legalon-SIL (LS). The cells were harvested after 2, 6, and 24 h after treatment. The levels of hepatitis C virus (HCV) RNA [core region (A) and NS5A region (B)] were quantified using qRT-PCR as described in “Materials and Methods”. The amounts of HCV RNA were normalized to glyceraldehyde-3-phosphate dehydrogenase. A: LS 50 μmol/L downregulated HCV RNA (core region) after 24 h. LS 200 μmol/L downregulated HCV RNA (core region) after 6 and 24 h; B: LS 50 μmol/L downregulated HCV RNA (NS5A region) after 24 h. LS 200 μM downregulated HCV RNA (core region) after 6 and 24 h. Data for RNA levels are mean ± SE (n = 3 independent experiments). ^aP < 0.05, ^bP < 0.01 vs DMSO control.