Epigallocatechin gallate inhibits HBV DNA synthesis in a viral replication - inducible cell line

Figure 6 Epigallocatechin gallate did not inhibit hepatitis B virus pregenomic RNA transcription. When doxycycline was removed from the cell culture medium, different concentrations of Epigallocatechin gallate (EGCG) were added to the HepG2.117 cells. After 3 d of culture, total RNA were extracted from the treated cells and the pregenomic RNAs (pgRNAs) were analyzed by semi-quantitative reverse transcription polymerase chain reaction. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs were used for normalization. NC: Total RNA sample was subjected to polymerase chain reaction without reverse transcription.