Epigallocatechin gallate inhibits HBV DNA synthesis in a viral replication - inducible cell line

Figure 3 Epigallocatechin gallate inhibited the production of hepatitis B virus precore mRNA. Twenty four hours after seeding, HepG2.117 cells were treated with medium containing different concentration of Epigallocatechin gallate (EGCG) for 3 d. Total RNAs were prepared and were quantitated by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) with a pair of precore mRNA selective primers. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was semi-quantitatively RT-PCR amplified and used for normalization. NC: Total RNA sample was subjected to PCR without reverse transcriptase and used as the negative control.