Polymorphisms of interleukin-10 promoter are not associated with prognosis of advanced gastric cancer

Table 1 Interleukin-10 promoter polymorphism identified by PCR-RFLP analysis

Polymorphism	Primer sequence	Annealing temperature	Restriction enzyme	Allele size
IL-10-1082G/A	5′-CTCGCTGCA ACCCAACTGGC-3′	58°C	MnlI	A: 139 bp
	5′-TCTTACCTATCCCTACTTCC-3′			G: 106, 33 bp
IL-10-819C/T	5′-TCATTCTATGTGCTGGAGATGG-3′	59°C	Mae III	C: 125, 84 bp
	5′-TGGGGGAAGTGGGTAAGAGT-3′			T: 209 bp
IL-10-592C/A	5′-GTGAGCACTACCTGACTAGC-3′	58°C	RsaI	C: 412 bp
	5′-CCTAGGTCACAGTGACGTGG-3′			A: 175, 237 bp