MiRNA profile in esophageal squamous cell carcinoma: Downregulation of miR-143 and miR-145

Figure 5 miR-143 and miR-145 inhibited esophageal squamous cell carcinoma cell mobility. A: The expression levels of miR-143 and miR-145 in esophageal squamous cell carcinoma (ESCC) cell lines. Using normal tissue as the control, both miRNAs were significantly downregulated in ESCC cell lines; B: The increased expression of miR-143 and miR-145 following stable transfection. Quantitative reverse transcription-polymerase chain reaction demonstrated a significant increase in the expression levels of miR-143 and miR-145 in KYSE150 and KYSE180 cells transfected with the respective expression plasmids compared with the levels in control cells (^bP < 0.01); C, D: The increased expression of miR-143 and miR-145 inhibited the mobility of both KYSE150 and KYSE180 cells. The miRNA stably transfected cells were seeded in a 6-well plate, streaks were made using a tip when the cells were grown to almost confluence 24 h later. Streaks were photographed at different intervals at 50 ×. This showed that upregulation of miR-143 and miR-145 can prevent ESCC cell wound healing. These findings are shown in (C) and (D).