Omeprazole induces gastric transmucosal permeability to the peptide bradykinin

Figure 3 ³H-bradykinin (panel A) or ³H-oxytocin (panel B) was added to the mucosal fluid compartment at t = 0 to begin the flux period of 180 min. Omeprazole or DMSO was added to the tissue 90 min after the addition of ³H-peptide. Samples were taken every 45 min for the total 180 min flux period (pre- and post-omeprazole/DMSO) from the serosal fluid compartment for LSC and TLC. Omeprazole increases the rate at which total radioactivity crosses the gastric mucosa compared to the paired vehicle control (DMSO) in a ³H-bradykinin flux experiment (panel A) whereas total radioactivity crossing the tissue in a ³H-oxytocin flux experiment (panel B) remains unchanged after the addition of omeprazole or DMSO. Both panel A and panel B represent two separate experiments and animals.