Involvement of PI3K and ERK1/2 pathways in hepatocyte growth factor-induced cholangiocarcinoma cell invasion

Figure 5 HGF induction of ERK1/2 and Akt phosphorylation in cholangiocarcinoma HuCCA-1 and KKU-M213 cell lines. About 80% confluent cells were treated with 50 ng/mL HGF in serum-free medium for 15, 60, 360 min. Lysates from HuCCA-1 (A) and KKU-M213 (B) cells were assessed for total and phosphorylated forms of ERK1/2 and Akt by Western blotting assay. The graphs showed band densities of phospho-ERK1/2 and phospho-Akt relative to those at zero time points. Data are presented as mean ± SE of results obtained from three independent experiments. ^aP < 0.05 vs untreated control.