Involvement of PI3K and ERK1/2 pathways in hepatocyte growth factor-induced cholangiocarcinoma cell invasion

Figure 3 Effects of HGF on E-cadherin expression and localization. A: cholangiocarcinoma (CCA) cells were treated with HGF for 6 h, then cell lysate was analyzed by Western blotting with anti-E-cadherin and -β-actin monoclonal antibodies; B: After treatment with 0 and 100 ng/mL HGF for 6 h, cells were analyzed by immunofluorescence using anti-E-cadherin antibody and visualized under confocal laser scanning microscopy (60 × objective magnification plus 2 × digital magnification).