Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats

Figure 4 Western blotting analysis of caspase-3 and Bcl-2 in hepatocytes. A: Compared with control values [normal control (NC) group, lane 1], the expression levels of Bcl-2 protein decreased in the phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt)-blocker and high-fat groups (lanes 2-4). In contrast, caspase-3 expression was equally elevated in each of the three treatment groups compared with the control value; B, C: Band density values are compared with those of the β-actin loading control. ^aP < 0.05 vs NC group.