Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats

Figure 1 Western blotting analysis of phosphatidylinositol 3-kinase and protein kinase B in hepatocytes. A: Western blotting analysis of total phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt). The protein expression levels of total PI3K and Akt showed no significant difference among the four groups; B: Western blotting analysis of phosphorylated PI3K and Akt. Left: In high-fat fed groups (HFD), the protein expression levels of both phosphorylated PI3K and Akt decreased compared with the normal control (NC) group. In PI3K blocker groups (NC + LY), pPI3K and pAkt showed no expression. In AKT blocker groups (NC + AI), pPI3K was normally expressed and pAkt showed no expression. Right: Band density values are compared with those of the β-actin loading control. ^a,cP < 0.05 vs NC group.