Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats

Table 1 Weights of the liver and serum levels of biochemical parameters in high-fat fed rats

	NC	NC + LY	NC + AI	HFD
Tissue weight
Liver (g)	1.55 ± 0.14	1.99 ± 0.32a	1.89 ± 0.43a	3.98 ± 0.64a
Serum
TG (mg/dL)	183.8 ± 70.4	396.7 ± 72.3	376.7 ± 68.4	589 ± 98.4b
ALT (U/L)	34 ± 3.1	86 ± 5.58a	89 ± 5.2a	207 ± 35.5b
AST (U/L)	36 ± 3.4	88 ± 6.1	99 ± 5.2a	187 ± 35.5b
GGT (U/L)	52 ± 6.4	48 ± 3.9	62 ± 4.4	232 ± 67.8b
Glucose (mmol/L)	5.1 ± 0.4	6.1 ± 0.5	5.7 ± 0.4	7.1 ± 71.3a
Insulin (mIu)	4.7 ± 0.7	14.9 ± 2.0b	15.0 ± 2.9b	21.0 ± 3.8b
Liver
IRI (HOMA-IR)	1.06	4.03a	3.8a	6.6b
TG (mg/g)	103. 1 ± 12.6	324.6 ± 13.4	2336.8 ± 11.6	421.5 ± 19.7b

Rats were fed and subjected to each measurement. Data are presented as the mean ± SD.

^aP < 0.05,

^bP < 0.01 vs rats fed with normal diets. NC: Normal control; NC + LY: NC plus the PI3K blocker LY294002; NC + AI: NC plus the AKT blocker; HFD: High-fat diets for 16 wk (n = 10^-12); ALT: Alanine aminotransferase; AST: Aspartate aminotransferase. GGT: γ-glutamyltransferase; TG: Triglycerides; IRI (HOMA-IR): Insulin resistant index.