Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients

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Dec 21, 2010 (publication date) through Jul 17, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 21, 2010; 16(47): 5965-5974
Published online Dec 21, 2010. doi: 10.3748/wjg.v16.i47.5965

Table 1 Primer sequences used for reverse transcription polymerase chain reaction amplification of target transcripts

Tumor marker	Sense	Antisense	PCR product size (bp)	Ref.
CEA	5'-GGGCCACTGTCGGCATCATGATTG-3'	5'-TGTAGCTGTTGCAAATGCTTTAAGGAAGAAGC-3'	131	[38]
CK20	5'-CAGACACACGGTGAACTATGG-3'	5'-GATCAGCTTCCACTGTTAGACG-3'	371	[39]
EGFR	5'-TCTCAGCAACATGTCGATGG-3'	5'-TCGCACTTCTTACACTTGCG-3'	474	[40]

CEA: Carcinoembryonic antigen; CK20: Cytokeratin 20; EGFR: Epidermal growth factor receptor; PCR: Polymerase chain reaction.

Citation: Tsouma A, Aggeli C, Lembessis P, Zografos GN, Korkolis DP, Pectasides D, Skondra M, Pissimissis N, Tzonou A, Koutsilieris M. Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients. World J Gastroenterol 2010; 16(47): 5965-5974
URL: https://www.wjgnet.com/1007-9327/full/v16/i47/5965.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i47.5965