Pro12Ala polymorphism of the peroxisome proliferator-activated receptor γ2 in patients with fatty liver diseases

Table 2 Real-time polymerase chain reaction primers and probes

Name	Oligonucleotide sequence	PCR application
Plas A primer	5'-CCGCTCTAGAACTAGTGAAGGAA-3'	Reference DNA
Plas S primer	5'-ACTCACTATAGGGCGAATTGG-3'
PPARγ A primer	5'-TTACCTTGTGATATGTTTGCAGACA-3'	Target DNA
PPARγ mis primer	5'-GTTATGGGTGAAACTCTTGGAGA-3'
TM LNA probe wt	5'-6FAM-CTATTGACCCAGAAAGC--BHQ1	Target and reference DNA
TM LNA probe mut	5'-YAK-CTATTGACGCAGAAAGC--BHQ1

Bold letters indicate the single nuclear polymorphism and underlined letters indicate the insertion of locked nucleotides (LNA). PPARγ: Peroxisome proliferator-activated receptor-γ; PCR: Polymerase chain reaction.