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©2010 Baishideng Publishing Group Co.
World J Gastroenterol. Dec 14, 2010; 16(46): 5815-5821
Published online Dec 14, 2010. doi: 10.3748/wjg.v16.i46.5815
Published online Dec 14, 2010. doi: 10.3748/wjg.v16.i46.5815
Table 2 Primers used in polymerase chain reaction for genotyping Toll-like receptor 9 polymorphisms and three single nucleotide polymorphisms
| Fragment (product) | Forward | Reverse |
| TLR9 polymorphisms | ||
| -2109 to -1293 (817 bp) | 5'-CCAAGGGACTCTGGGAAAG-3' | 5'-CATGTCACCCTCTCAACAGGG-3' |
| -1626 to -1095 (532 bp) | 5'-CAGCCTTCACTCAGAAATACCC-3' | 5'-GGCCAACAAGGCCCTATG-3' |
| -1296 to -553 (662 bp) | 5'-CATGGGAGCAGAGACATAATG-3' | 5'-GCCAGGGTGTAGCTTGA-3' |
| -795 to -134 (662 bp) | 5'-GAGTCTCCTCACCTAGATCAG-3' | 5'-TATACCAGCCTAGTAGC-3' |
| 79 to 962 (884 bp) | 5'-CTGCAAGCAACAGTGACGG-3' | 5'-AGCTTTCACTTAACCAATCCC-3' |
| -393 to 1698 (2092 bp) | 5'-TACCCGCTACTGGTGCTATC-3' | 5'-TGGCAGAGTCTAGCATCAGG-3' |
| 996 to 1687 (692 bp) | 5'-CTGGTTCTGAAGCCTAATTC-3' | 5'-AGCATGAGGATGTTGGTATGG-3' |
| 976 to 1899 (924 bp) | 5'-CTGGATTCTAGGTCTCAGTCC-3' | 5'-CTGGATTCTAGGTCTCAGTCC-3' |
| 1540 to 2368 (829 bp) | 5'-CCTGCCACATGACCATCGAG-3' | 5'-CCTGCCACATGACCATCGAG-3' |
| 1812 to 2520 (709 bp) | 5'-AACCTCACCCACCTGTCAC-3' | 5'-AACCTCACCCACCTGTCAC-3' |
| 2407 to 3350 (944 bp) | 5'-TGCAGATGAACTTCATCAACC-3' | 5'-GCTGTTGCAGCTGACATC-3' |
| 3267 to 4057 (791 bp) | 5'-CAGGAAACCAGCTGAAGG-3' | 5'-CAGGAAACCAGCTGAAGG-3' |
| 3867 to 4701 (835 bp) | 5'-GACTGGGTGTACAACGAGCTT-3' | 5'-TTCTGCATGGGAAAGGTAGG-3' |
| SNP | ||
| -1486 | 5'-CAGCCTTCACTCAGAAATACCC-3' | 5'-GGCCAACAAGGCCCTATG-3' |
| 1174 | 5'-CTGGTTCTGAAGCCTAATTC-3' | 5'-AGCATGAGGATGTTGGTATGG-3' |
| 2848 | 5'-TGCAGATGAACTTCATCAACC-3' | 5'-GCTGTTGCAGCTGACATC-3' |
- Citation: Fuse K, Katakura K, Sakamoto N, Ohira H. Toll-like receptor 9 gene mutations and polymorphisms in Japanese ulcerative colitis patients. World J Gastroenterol 2010; 16(46): 5815-5821
- URL: https://www.wjgnet.com/1007-9327/full/v16/i46/5815.htm
- DOI: https://dx.doi.org/10.3748/wjg.v16.i46.5815