Screening compounds against HCV based on MAVS/IFN-β pathway in a replicon model

Figure 3 Secreted placental alkaline phosphatase reporter correlates with the anti-hepatitis C virus activity of interferon-α. A, B: Increase of secreted placental alkaline phosphatase (SEAP) activity in enhanced yellow fluorescent protein (eYFP)-mitochondrial antiviral signaling protein (MAVS) and interferon (IFN)-β-SEAP co-transfected Huh7.5 (P > 0.05) and replicon cells (P < 0.05) treated with IFN-α. Before transfection, Huh7.5 and replicon cells were incubated in the absence or presence of 20, 50, 100 and 200 IU/mL IFN-α for 72 h. SEAP activity was measured at 48 h post-transfection in the presence of IFN-α. The percentage increase in luciferase activity relative to the untreated controls was plotted. Bars indicate SD (n = 3).