Chronic stress sensitizes rats to pancreatitis induced by cerulein: Role of TNF-α

Table 3 Inflammatory parameters in pancreatic tissue (mean ± SE)

Group	TNF-α (ng/mg DNA)	HIF-1α (ng/mg DNA)	Active trypsin (nmol/mg DNA)	NF-κB (p65) (μg/mg DNA)	NF-κB (p50) (μg/mg DNA)	MPO (U/mg DNA)	LPO (nmol/mg DNA)	MDA (μmol/mg DNA)
Stress (-)
Veh	BD	BD	0.11 ± 0.02	43 ± 5	0.48 ± 0.04	0.00 ± 0.00	BD	0.71 ± 0.03
Cer	BD	BD	0.23 ± 0.04	46 ± 6	0.51 ± 0.05	0.00 ± 0.00	BD	0.89 ± 0.05
Anti-TNF-α + Cer	BD	BD	0.22 ± 0.05	44 ± 3	0.50 ± 0.03	0.00 ± 0.00	BD	0.84 ± 0.05
Stress (+)
Veh	1.6 ± 0.2a	0.9 ± 0.1a	0.37 ± 0.06	72 ± 6	0.84 ± 0.07	1.41 ± 0.07	27 ± 2	2.05 ± 0.11
Cer	5.4 ± 0.3a	3.6 ± 0.3a	4.15 ± 0.32a	389 ± 25a	5.54 ± 0.35a	15.90 ± 0.66a	316 ± 20a	22.16 ± 0.69a
Anti-TNF-α + Cer	2.0 ± 0.2c	1.5 ± 0.3c	1.79 ± 0.21c	221 ± 18c	2.65 ± 0.24c	7.14 ± 0.38c	171 ± 14c	12.38 ± 0.47c

^aP < 0.05 vs control-vehicle Stress (-) group;

^cP < 0.05 vs Stress (+) plus Cer group. BD: Below detection limit; TNF-α: Tumor necrosis factor-α; HIF-1α: Hypoxia inducible factor-1α; NF-κB: Nuclear factor-κB; MPO: Myeloperoxidase; LPO: Lipid hydroperoxide; MDA: Malondialdehyde.