HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow

Figure 6 Detection of P450 genes in hepatocyte nuclear factor-4α overexpressing induced mesenchymal stem cells by real-time reverse transcription-polymerase chain reaction. The induced mesenchymal stem cells (8 wk post-induction) were infected with adenovirus containing rHNF-4α gene (indicated as AdIEHNF4) or parental control adenovirus (indicated as AdIE). The infected cells were further treated with inducer B-naphthoflavone (indicated as AdIE/b or AdIEHNF4/b) or rifampicin (indicated as AdIE/R or AdIEHNF4/R) for 2 d. The relative level of each gene in the control group was set to 1. The induction effect of HNF-4α and/or inducer on the expression of P450 genes is represented as (A) CYP1A1, CYP1A2, and CYP2C19; (B) CYP2B6, CYP2C9, and CYP3A4; (C) CYP2A6, CYP2C8, CYP2D6, and CYP2E1, respectively. The amount of input RNA was normalized using the HPRT gene. Each column represents the mean ± SD of three independent experiments and the induction fold by HNF-4α overexpression for each gene was significant (P < 0.05). HNF: Hepatocyte nuclear factor.