HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow

Figure 5 Detection of liver-specific genes in hepatocyte nuclear factor-4α overexpressing induced mesenchymal stem cells by real-time reverse transcription-polymerase chain reaction. The induced mesenchymal stem cells (8 wk post-induction) were infected with adenovirus containing rHNF-4α gene (indicated as AdIEHNF4) or parental control adenovirus (indicated as AdIE). The relative level of each gene in the control group was set to 1. The induction effect of HNF-4α on the expression of liver-specific genes was represented as Albumin, tyrosine-aminotransferase (TAT), and glucose 6-phosphatase (G6P), respectively. The amount of input RNA was normalized using the hypoxanthine-guanine phosphoribosyltransferase gene. Each column represents the mean ± SD of three independent experiments and the induction fold for each gene was significant (P < 0.05). HNF: Hepatocyte nuclear factor.