HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow

Figure 3 Detection of hepatocyte nuclear factor-4 isoforms in induced mesenchymal stem cells and human hepatoma cell lines by real-time reverse transcription-polymerase chain reaction. A: The relative level of hepatocyte nuclear factor (HNF)-4γ in induced mesenchymal stem cells (MSCs) at the indicated time point (0, 1, 4, 7 and 10 wk post-induction), HA22T/VGH and SK-Hep-1 cells; B: The relative level of HNF-4α in induced MSCs at the indicated time point (0, 1, 4, 7 and 10 wk post-induction) and HepG2 cells. The relative level at 0 wk was set to 1. The amount of input RNA was normalized using the housekeeping gene β2M. Each column represents the mean ± SD of three independent experiments.