Daily genetic profiling indicates JAK/STAT signaling promotes early hepatic stellate cell transdifferentiation

Figure 3 Gene expression markers during different stages of hepatic stellate cells activation. A: Markers of quiescence, early and late activation, fibrosis and the IL-6 signaling pathway (A-E, respectively) were examined over 10 d in culture. Real time polymerase chain reaction was used to analyze gene expression. Fold induction was relative to day Q expression levels. Data are presented as mean ± SE. IL-6: Interleukin 6; IL-6R: Interleukin 6 receptor; SOCS: Suppressors of cytokine signaling protein; TGF: Transforming growth factor; STAT: Signal transducers and activators of transcription; PPAR: Peroxisome proliferator activated receptor; JAK: Janus kinase 2; SMA: Smooth muscle α-actin; MMP: Matrix metalloproteinase.