Daily genetic profiling indicates JAK/STAT signaling promotes early hepatic stellate cell transdifferentiation

Figure 1 Gene expression profile time course. A: Mini-array of 21 genes across 10 d in culture as analyzed by real time polymerase chain reaction. Results are averages from three separate experiments. Quiescent time point was set to 1 for all genes; B: Classic housekeeping markers (G3PDH, β-actin and HPRT) fluctuated over days in culture activation, thus they served as unreliable normalization markers. IL-6: Interleukin 6; IL-6R: Interleukin 6 receptor; SOCS: Suppressors of cytokine signaling protein; TGF: Transforming growth factor; STAT: Signal transducers and activators of transcription; PPAR: Peroxisome proliferator activated receptor; FAK: Focal adhesion kinase; JAK: Janus kinase 2; SMA: Smooth muscle α-actin; MMP: Matrix metalloproteinase.