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©2010 Baishideng Publishing Group Co.
World J Gastroenterol. Oct 28, 2010; 16(40): 5092-5103
Published online Oct 28, 2010. doi: 10.3748/wjg.v16.i40.5092
Published online Oct 28, 2010. doi: 10.3748/wjg.v16.i40.5092
Table 2 Primer sets for real-time reverse transcription-polymerase chain reaction analysis
Gene | Forward primer | Reverse primer | Probe |
HNF-1α | TGAGTCCGGGCTTCACAC | GGCTGCTGGAGGACACTG | 42 |
HNF-3β | GCACCTGCAGATTCTGATTTT | GACTTCCCTGCAACAACAGC | 25 |
HNF-4α | ATTGACAACCTGTTGCAGGA | CGTTGGTTCCCATATGTTCC | 77 |
HNF-4γ | CTTGGTGGAATGGGCTAAA | AGCTCTCAACAGTGCCACCT | 8 |
HNF-6 | CCTGGAGCAAACTCAAATCC | TTCTTTCCTTTTGCATGCTG | 88 |
C/EBPα | CAACACTTGTATCTGGCCTCTG | CGAGCAAAACCAAAACAAAAC | 3 |
PPARα | GTCCCCGCAGATTCTACATT | GAAGCTGTCCTGGCTCAGAT | 14 |
Alb | TGTTGCCAAGCTGCTGATA | CCTTCATCCCGAAGTTCATC | 27 |
TAT | TGCTGAGCAGTCTATCCACT | CTGCTCACAGAACTCCTGGAT | 67 |
G6P | GCTGCTCATTTTCCTCATCAA | TTCTGTAACAGCAATGCCTGA | 67 |
CYP1A1 | GACAGATCCCATCTGCCCTA | CAAACTTGTGTCTCTTGTTGTGC | 80 |
CYP1A2 | CAAGAAATGCTGTGTCTTCGTAA | AGAGGGGTCCTCCCACAG | 59 |
CYP2A6 | CAAAAAGGACACCAAGTTTCG | AGAGCCCAGCATAGGGTACA | 69 |
CYP2B6 | GAAGCTTTTATCCCCTTCTCCT | GCCATGGAGAAGTTCTGGAG | 35 |
CYP2C8 | AAGAAAAGTGACTACTTCATGCCTTT | CAAGTCCTTCTCCTGCACAA | 18 |
CYP2C9 | ATTGACCTTCTCCCCACCA | CAGGGAAATTAATATGGTTGTGC | 43 |
CYP2C19 | GTCCAGAGATACATCGACCTCA | AGTGAGGGAAGTTAATATGGTTGTG | 43 |
CYP2D6 | AGGAGGAGTCGGGCTTTCT | CGCTGGGATATGCAGGAG | 56 |
CYP2E1 | CAAGCCATTTTCCACAGGA | CAACAAAAGAAACAACTCCATGC | 67 |
HPRT | AGGTTGCAGCGAGGAAAAA | AGCCACCCTGGAAGAAACTT | 59 |
- Citation: Chen ML, Lee KD, Huang HC, Tsai YL, Wu YC, Kuo TM, Hu CP, Chang C. HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow. World J Gastroenterol 2010; 16(40): 5092-5103
- URL: https://www.wjgnet.com/1007-9327/full/v16/i40/5092.htm
- DOI: https://dx.doi.org/10.3748/wjg.v16.i40.5092