Clinical relevance of Helicobacter pylori babA2 and babA2/B in Costa Rica and Japan

Table 1 PCR primers and conditions for detection of babA2, babB and babA2/B genes

Region	Primer	Nucleotide sequence (5’- 3’)	PCR conditions	Ref.
babA2	babA-F	AATCCAAAAAGGAGAAAAAGTATGAAA		[4]
	babA-R	TGTTAG TGATTTCGGTGTAGGACA
	babA2-Fnc1	GAAAAAACATGAAAAAACACATCCTTTCAT		[16]
	babA2-Rmn2	TCTGGGTTAATGGCTTGCC
babB	babB-Fnc1	CTCTCTCTCGTTTTTGCTCCA	96°C for 2 min, 30 cycles (96°C for 30 s, 48°C for 30 s, 72°C for 1 min)	This study
	babB-Rnc1	CTTCATAACACACCCCTAAAGAGTC
	babB-Fnc3	ATGAAAAAAACCCTTTTACTC	96°C for 2 min, 30 cycles (96°C for 30 s, 46°C for 30 s, 72°C for 1 min)	This study
	babB-Rnc3	TGACCTGGATTGGTGCCCCTACG
babA2/B	babA-F	AATCCAAAAAGGAGAAAAAGTATGAAA		[4]
	babB-Rnc1	CTTCATAACACACCCCTAAAGAGTC
	babA2-Fnc1	GAAAAAACATGAAAAAACACATCCTTTCAT		[16]
	babB-Rnc1	CTTCATAACACACCCCTAAAGAGTC	96°C for 2 min, 30 cycles (96°C for 30 s, 62°C for 30 s, 72°C for 1 min)	This study
	babB-Rnc21	CTACGCTCACCCCCTTGTACTTC	96°C for 2 min, 30 cycles (96°C for 30 s, 63°C for 30 s, 72°C for 1 min)	This study
	babB-Rnc31	TGACCTGGATTGGTGCCCCTACG	96°C for 2 min, 30 cycles (96°C for 30 s, 62°C for 30 s, 72°C for 1 min)	This study

¹Used with babA2-Fnc1.