HBx-induced reactive oxygen species activates hepatocellular carcinogenesis via dysregulation of PTEN/Akt pathway

Figure 1 Effect of hepatitis B virus X-protein on induction of aberrant cell growth. A: Cell growth analysis by crystal violet staining and A_{550 nm} detection. 10⁴ cells were seeded, and Stained at 1, 5, 6, 7 and 8 d after seeding. Values represent mean ± SD (n = 3). ^bP≤ 0.001 compared with mock transfectants; B: Morphology of the cells was observed by optical microscopy. HBx: Hepatitis B virus X-protein.