Lowered HGK expression inhibits cell invasion and adhesion in hepatocellular carcinoma cell line HepG2

Figure 2 Hepatocyte progenitor kinase-like kinase expression suppressed by RV-shHGK-1 retrovirus in HepG2 cells. A: HepG2 cells infected with retrovirus mediating RNAi targeting of hepatocyte progenitor kinase-like kinase (RV-shHGK)-1 or RV-shHGK-C (multiplicity of infection = 4), GFP expression and the phase contrast images after 48 h (original magnification × 200); B: Protein and mRNA levels of hepatocyte progenitor kinase-like kinase (HGK) after HepG2 cells were treated with different retrovirus detected by real-time reverse transcriptase polymerase chain reaction and Western boltting assay. The retrovirus RV-shHGK-1 significantly inhibited HGK expression in HepG2 cells, ^aP < 0.05 vs HepG2 cells and HepG2 cells infected with RV-shHGK-C retrovirus.