Lowered HGK expression inhibits cell invasion and adhesion in hepatocellular carcinoma cell line HepG2

Figure 1 Selection of the most effective hepatocyte progenitor kinase-like kinase specific shRNA expression vector in 293T cells. A: Phase contrast and GFP expression under a fluorescent microscope after 48 h in 293T cells; B: Protein mRNA and hepatocyte progenitor kinase-like kinase (HGK) levels after HepG2 cells were treated with different vectors detected by real-time reverse transcriptase polymerase chain reaction and Western boltting assay. The vector retrovirus mediating RNAi targeting of HGK (RV-shHGK)-1 significantly inhibited HGK expression in HepG2 cells, ^aP < 0.05 vs HepG2 cells and HepG2 cells transfected with RV-shHGK-C vector. 1: 293T cells; 2: Transfection of RV-shHGK-1 vector in 293T cells; 3: Transfection of RV-shHGK-2 vector; 4: Transfection of RV-shHGK-3 vector; 5: Transfection of RV-shHGK-C vector (original magnification × 200).