Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome

Table 5 Number of 16S rRNA gene copies detected in 50 ng of fecal DNA

qPCR assay	All (n = 44)	BMI < 251 (n = 17)	BMI > 25 (n = 22)	P2
Bifidobacterium catenulatum/Pseudocatenulatum-like	4.8 (1.6)3	5.4 (0.7)	4.3 (1.8)	0.366
Bifidobacterium spp.	5.8 (1.0)	5.5 (1.0)	6.2 (0.9)	0.009
Clostridium coccoides/Eubacterium rectale-group	7.3 (0.2)	7.3 (0.1)	7.3 (0.2)	0.630
Clostridium cocleatum 88%	5.6 (1.1)	5.5 (1.4)	5.6 (1.0)	0.483
Collinsella aerofaciens-like	5.6 (1.1)	5.3 (1.2)	5.8 (0.9)	0.294
Coprococcus eutactus 97%	5.3 (1.5)	5.5 (1.7)	5.4 (1.3)	0.639
Desulfovibrio desulfuricans-group	3.9 (0.8)	3.8 (0.6)	4.0 (0.9)	0.403
Eubacterial 16S	6.0 (0.3)	5.9 (0.4)	6.0 (0.3)	0.630
Lactobacillus-group	3.9 (0.9)	4.2 (0.7)	3.7 (0.9)	0.060
Ruminococcus torques 91%	4.6 (0.8)	4.4 (0.8)	4.8 (0.7)	0.461
Ruminococcus torques 94%	3.8 (1.0)	4.0 (1.3)	3.6 (0.8)	0.409
Streptococcus bovis-like	2.7 (1.5)	3.2 (2.1)	2.5 (1.2)	0.732
Veillonella spp.	3.4 (1.0)	3.5 (1.1)	3.4 (1.1)	0.745

¹Body mass index (BMI) data missing for 5 subjects;

²Calculated with Mann-Whitney U-test;

³Values are presented as log10 averages with standard deviation in parentheses. qPCR: Quantitative real-time polymerase chain reaction.