Paeonol inhibits tumor growth in gastric cancer in vitro and in vivo

Figure 3 Induction of apoptosis by paeonol in cultured mouse forestomach carcinoma and SGC-7901 cells. A: Paeonol increased the rate of apoptosis, as determined by the number of cells with sub-G1 DNA content by flow cytometry. Values are expressed as the mean ± SD from three replicate experiments. ^aP < 0.05, ^bP < 0.01, paeonol treated vs untreated control; B, C: Effects of paeonol on apoptosis of gastric cancer cell line SGC-7901 (B) and mouse forestomach carcinoma (MFC) cells (C) by flow cytometry. One representative flow cytometry tracing was shown from three replicate experiments. AP: Apoptotic percentage; D: Fluorescent staining of nuclei (× 320) in paeonol-treated and untreated cells by acridine orange. SGC-7901 and MFC cells treated with 62.5 mg/L paeonol for 48 h. Condensed and fragmented nuclei and apoptotic bodies were seen in the paeonol-treated cells, but not in the control.