Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome

Figure 1 Classification accuracy is dependent on amplicon size. Full length sequences were trimmed to 900, 800, 700, 600, 500, 400, 350, 300, 200,100, and 50 bases with each amplicon starting at Escherichia coli base 28. Each sequence trim was uploaded onto Ribosomal Database ProjectII classifier and the results at each taxonomical level were compared to results obtained using the full length sequence. Percent classification accuracies at the genus level for each amplicon size trim are shown.