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World J Gastroenterol. Sep 7, 2010; 16(33): 4135-4144
Published online Sep 7, 2010. doi: 10.3748/wjg.v16.i33.4135
Published online Sep 7, 2010. doi: 10.3748/wjg.v16.i33.4135
Table 6 Taxonomic coverage of domain bacteria by primers 347F and 803R
| Primer | Optimization | Total species | Coverage at mismatches n (%) | Total sequence | Coverage at mismatches n (%) | ||||
| 0 | 1 | 2 | 0 | 1 | 2 | ||||
| 374F | Before | 5165 | 3392 (65.7) | 4835 (93.6) | 5042 (97.6) | 433 306 | 275 801 (63.7) | 406 626 (93.8) | 418 613 (96.6) |
| After | 5165 | 4703 (91.1) | 4996 (96.7) | 5114 (99.0) | 433 306 | 391 695 (90.4) | 418 832 (96.7) | 424 756 (98.0) | |
| 803R | Before | 5165 | 4584 (88.8) | 5091 (98.6) | 5159 (99.9) | 433 306 | 352 827 (81.4) | 417 612 (96.4) | 430 967 (99.5) |
| After | 5165 | 4741 (91.8) | 5131 (99.3) | 5159 (99.9) | 433 306 | 367 771 (84.9) | 427 791 (98.7) | 431 725 (99.6) | |
- Citation: Nossa CW, Oberdorf WE, Yang L, Aas JA, Paster BJ, DeSantis TZ, Brodie EL, Malamud D, Poles MA, Pei Z. Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. World J Gastroenterol 2010; 16(33): 4135-4144
- URL: https://www.wjgnet.com/1007-9327/full/v16/i33/4135.htm
- DOI: https://dx.doi.org/10.3748/wjg.v16.i33.4135