Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome

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Sep 7, 2010 (publication date) through Nov 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 7, 2010; 16(33): 4135-4144
Published online Sep 7, 2010. doi: 10.3748/wjg.v16.i33.4135

Table 5 Esophageal species misclassified using amplicon B’ compared with Sanger sequences

Species	Weight (of 6800)	Species identified by Amplicon B’
Actinomyces naeslundii	2	Actinomyces viscosus
Atopobium AY959044	8	Atopobium parvulum
Bacteroides vulgatus	1	Uncultured bacterium
Bradyrhizobium japonicum	1	Blastobacter denitrificans
Campylobacter showae	4	Campylobacter
Escherichia flexneri	2	Shigella sonnei
Haemophilus aegyptius	17	Haemophilus influenzae
Haemophilus haemolyticus	33	Haemophilus
Lactobacillus gasseri	2	Lactobacillus johnsonii
Leptotrichia wadeii	7	Leptotrichia shahii turn
Neisseria macaca	25	Uncultured bacterium
Prevotella melaninogenica	288	Uncultured bacterium
Pseudoramibacter	1	Uncultured bacterium
Streptococcus infantis	77	Streptococcus mitis
Total	468

Citation: Nossa CW, Oberdorf WE, Yang L, Aas JA, Paster BJ, DeSantis TZ, Brodie EL, Malamud D, Poles MA, Pei Z. Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. World J Gastroenterol 2010; 16(33): 4135-4144
URL: https://www.wjgnet.com/1007-9327/full/v16/i33/4135.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i33.4135