Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome

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Sep 7, 2010 (publication date) through Nov 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 7, 2010; 16(33): 4135-4144
Published online Sep 7, 2010. doi: 10.3748/wjg.v16.i33.4135

Table 4 Foregut species misclassified using amplicon B’ compared with full length sequences

Species	Weight (of 9484)	Species identified using amplicon B
Atopobium AY959044	8	Atopobium parvulum
Bacteroides vulgatus	1	Uncultured bacterium
Bradyrhizobium japonicum	0	Blastobacter denitrificans
Bradyrhizobium liaoningense	1	Blastobacter denitrificans
Escherichia fergusonii	1	Shigella sonnei
Escherichia flexneri	4	Shigella sonnei
Haemophilus aegyptius	17	Haemophilus influenzae
Haemophilus haemolyticus	34	Haemophilus
Lactobacillus gasseri	2	Lactobacillus johnsonii
Leptotrichia wadeii	10	Leptotrichia shahii
Neisseria macaca	28	Uncultured bacterium
Prevotella melaninogenica	369	Uncultured bacterium
Pseudoramibacter	3	Uncultured bacterium
Streptococcus infantis	193	Streptococcus mitis
Total	671

Citation: Nossa CW, Oberdorf WE, Yang L, Aas JA, Paster BJ, DeSantis TZ, Brodie EL, Malamud D, Poles MA, Pei Z. Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. World J Gastroenterol 2010; 16(33): 4135-4144
URL: https://www.wjgnet.com/1007-9327/full/v16/i33/4135.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i33.4135