Specific shRNA targeting of FAK influenced collagen metabolism in rat hepatic stellate cells

Figure 2 Focal adhesion kinase short hairpin RNA selectively inhibited the expressions of collagen I mRNA and collagen III mRNA in hepatic stellate cells after focal adhesion kinase short hairpin RNA transfection. A, B: Real-time polymerase chain reaction SYBR Green I fluorescence history vs cycle number of target gene 1 (collagen I, A) and target gene 2 (collagen III, B) in sample cDNA. The cycle threshold (Ct) is shown by the darker horizontal line; C, D: The relative quantification of collagen I mRNA (C) and collagen III mRNA (D) are calculated according to 2^-ΔΔC(t), [ΔΔCt = (Ct_{Collagen I or III} - Ct_GAPDH) experimental group - (Ct_{Collagen I or III} - Ct_GAPDH) control group] and shown in the bar graphs (n = 3, ^bP < 0.01 vs control, ^dP < 0.01 vs HK). It showed that the levels of type I collagen and type III collagen mRNA transcripts in fibronectin (FN) group was significantly higher than in the control group. FAK: Focal adhesion kinase; shRNA: Short hairpin RNA.