High expression of ErbB2 contributes to cholangiocarcinoma cell invasion and proliferation through AKT/p70S6K

Figure 6 Effects of ErbB2 inhibition (using siRNA and specific kinase inhibitor) on ERK1/2, AKT, and p70S6K phosphorylation in the cholangiocarcinoma cell lines. A: Cholangiocarcinoma cell lines were transfected with siErbB2 and siNeg, as a control, for 72 h and the collected cell lysates were analyzed by Western blotting; B: KKU-M213 cells were treated without and with AG825 in medium that contained 10% fetal bovine serum for 6 h, then lysed and analyzed by Western blotting. The results shown are representatives of three separate experiments.