High expression of ErbB2 contributes to cholangiocarcinoma cell invasion and proliferation through AKT/p70S6K

Figure 5 Effects of ErbB2 knockdown on cell invasion motility and proliferation of the three cholangiocarcinoma cell lines. Cells transfected with siRNA (negative control and ErbB2 targeted siRNA) for 72 h were analyzed for in vitro invasion (A) and motility (B) by using a Transwell chamber coated with and without Matrigel. After 6 h of incubation, numbers of invading/motile cells were counted. Cell proliferation (C) was analyzed by determining the number of viable cells during 24-96 h post-transfection by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Data are expressed as mean ± SE of the percentage relative to the controls obtained from three independent experiments. ^aP < 0.05, ^bP < 0.01 vs controls.