High expression of ErbB2 contributes to cholangiocarcinoma cell invasion and proliferation through AKT/p70S6K

Figure 1 Levels of ErbB2 expression in three cholangiocarcinoma cell lines and inhibition of ErbB2 phosphorylation by AG825. A: Cells cultured in 10% fetal bovine serum (FBS)-containing medium were lysed and the lysates were analyzed for ErbB2 mRNA by real-time reverse-transcriptase polymerase chain reaction. Data are presented as mean ± SE of ErbB2 mRNA level normalized with β-actin mRNA obtained from three independent experiments; B: Cells were incubated without and with 30 μmol/L AG825 in 10% FBS medium for 6 h, then lysed and analyzed for ErbB2, phospho-ErbB2 (Y1248) and β-actin by Western blotting. The results shown are representative of three separate experiments. ^bP < 0.01 vs HuCCA-1 and KKU-100.