Brief Article
Copyright ©2010 Baishideng.
World J Gastroenterol. Aug 21, 2010; 16(31): 3950-3956
Published online Aug 21, 2010. doi: 10.3748/wjg.v16.i31.3950
Figure 2
Figure 2 CC14 inhibits nucleotide triphosphatase affinity in nuclei of HepG2 cells. A: Methyl thiazolyl tetrazolium (MTT) metabolic viability assay at a dose of 10 mmol/L showing the inhibitory effect of CCl4 on growth of HepG2 cells; B: Substrate-affinity (Km, B2) is decreased without any alterations in the substrate binding sites (Vmax,B1) for adenosine triphosphate (ATP) or guanine triphosphate (GTP) in nuclear nucleotide triphosphatase of HepG2 cells treated with CCl4 (2 mmol/L); C: Enzyme’ activities of nuclei extracts and homogenates from HepG2 cells in control and CCl4 treatment groups. aP < 0.05 vs control group, or nuclear envelop group, respectively; bP < 0.01 vs their counterpart control group, or nuclear envelop group, respectively. LDH: Lactose dehydrogenase.