Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy

Figure 2 Characteristics of dipeptidyl peptidase IV-positive cells in the liver following transplantation of non-parenchymal cell preparations and hepatocytes. Frozen sections were obtained from recipient livers present at 16 wk post-transplantation. Acetone-fixed frozen sections were single or double-labeled by indirect immunofluorescence analysis with an antibody detecting the donor cell specific dipeptidyl peptidase IV (red) (A-C) and bile-duct specific anti-CD49f (green) (C). Merged images are combined with blue nuclear DAPI-staining. Circumscribed and compact clusters of hepatocyte repopulation could be distinguished from large areas of endothelial repopulation which covered a maximum of 90% of the section plane. Formation of bile duct-like structures could be detected by co-staining with the duct marker CD49f. PV: Portal vein.