Mapping of liver-enriched transcription factors in the human intestine

Figure 6 Electromobility shift assay with nuclear extracts of the human colon carcinoma Caco-2 cell line. A: Demonstrates the DNA binding of HNF6 to an optimized oligonucleotide probe using either nuclear protein extract of human liver tissue or Caco-2 cells. Note, the lane labeled as AB refers to the addition of a hepatocyte nuclear factor 6 antibody to demonstrate specificity of the DNA binding assay; B: Demonstrates the DNA binding of HNF4α to an optimized oligonucleotide probe using either nuclear protein extract of human liver tissue or Caco-2 cells. Note, the lane labeled as AB refers to the addition of a HNF4α antibody to demonstrate specificity of the DNA binding assay; C: Demonstrates the DNA binding of NGN3 (a HNF6 target gene) to an optimized oligonucleotide probe using either nuclear protein extract of human liver tissue or Caco-2 cells. Note, the lane labeled as × 100 or × 500 refers to competition assays with excess of unlabeled probe.