Germline mutation analysis of hPMS2 gene in Chinese families with hereditary nonpolyposis colorectal cancer

Table 2 Primer sequences and polymerase chain reaction condition of individual exon of hPMS2 gene

Exon	Primer sequence (5’-3’)	Size (bp)	AT (°C)	CN
1	M13F-ACGTCGAAAGCAGCCAATGGGAGTT	475	66	28
	M13R-CAGGTAGAAAGGAAATGCATTCAGT
2	M13F-ACAGTGTTGAGTCATTTCCCACAGT	455	66	28
	M13R-TTCTTAGCATAACACCTGCCTGGCA
3	M13F-TAGTCTGGGCTAGTAAATAGCCAGA	705	68	35
4	M13R-TATGACTTAGATTGGCAGCGAGACA
5	M13F-CTTGATTATCTCAGAGGGATCGTCA	540	68	35
	M13R-TCTCACTGTGTTGCCCAGTCCTAAT
6	M13F-TGCTTCCCTTGATTTGTGCGATGAT	504	67	32
	M13R-TGAGGCAGGAGAATTGCTTGAATCT
7	M13F-ACCCACGAGTTTGACATTGCAGTGA	498	60	35
	M13R-GTAGAGGTTGCAGTGAGCCAAGATA
8	M13F-AGATTTGGAGCACAGATACCCGTGA	414	61	32
	M13R-TGCGGTAGACTTCTGTAAATGCACA
9	M13F-CCTTCTAAGAACATGCTGGTTGGTT	279	64	45
	M13R-ATCTCATTCCAGTCATAGCAGAGCT
10	M13F-AGCCCTTCCGTATTTTGTCTATTCA	719	61	32
	M13R-GCTTTAGAAGCTGTTTGTACACTGT
11	M13F-TCACATAAGCACGTCCTCTCACCAT	1021	64	45
	M13R-GCAACAGAGCAAGACTCTGTCTCAA
12	M13F-GCCAAGATTGTGCCATTGCACTGTA	493	64	25
	M13R-AGTAGATACAAGGTCTTGCTGTGTT
13	M13F-GTGACACTTAGCTGAGTAGTGTTGT	372	64	35
	M13R-ATGTTAGCCAGGCTGGTCTCAAACT
14	M13F-GGTCTGTATCTCCTGACCTCATGAT	473	64	35
	M13R-GCACGTAGCTCTCTGTGTAAAATGA
15	M13F-GCTGAGATCTAGAACCTAGGCTTCT	522	64	35
	M13R-ACACACGAGCGCATGCAAACATAGA

AT: Anneal temperature; CN: Cycle number. The sequence of M13F was 5’-GTAAAACGACGGCCAGT-3’. The sequence of M13R was 5’-AACAGCTATGACCATG-3’.