Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates

Figure 1 Cloning of H. pylori hopE and hopV genes. A: Polymerase chain reaction (PCR) amplification of hopE and hopV genes. Amplicons and plasmids were separated by 1% agarose gel electrophoresis. Lane Std: 1 kb DNA ladder standard; B: Detection of plasmids carrying the hopE gene. Lane 1: Strain AD494(DE3)pLysS with pET21d as control. Lanes 2, 3: Negative clones, lanes 4-7 plasmids carrying the hopE gene; C: Release of inserts carrying the hopE and hopV genes after NcoI/HindIII and NdeI/BamHI digestions respectively. Lane Std: 1 kb DNA ladder standard (Fermentas).