Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates

Table 1 Primer sequences used for amplification and sequencing of H. pylori hopE and hopV genes

Name	5'→3' sequence	Restriction sites1
HopV12	GGGCCCATATGCTCAATTTTATGACAAAGAAGAAAAATAGAATGC	NdeI
HopV22	GGATCCCATGGTTAAAAATCCCTCAAGTAACTGATTTG	BamHI
HopE12	GGCGCCATGGAATTTATGAAAAAGTTTGTAGCTTTAGG	NcoI
HopE22	CGCGAAGCTTTTAAAAAGTGTAGTTATACCCTAAATAAAG	HindIII
HopE113	GCAAGTGGTTTGGTTTTAGAG	-
HopE223	ACCATATCCAACTGGATTTT	-
HopV113	GGCGTGGGGTTAGATACGCTG	-
HopV223	ACCATGTTTTCTTTATTCAC	-
HopVint3	ATGCGTTATTATGGGTTTTTTGACT	-
pETT7d4	TAATACGACTCACTATAGGG	-
pETT7r4	GCTAGTTATTGCTCAGCGG	-

¹The restriction sites included in primer sequences and used for ligation to plasmid vectors are shown underlined;

²External primers used to clone porin genes;

³Internal primers used for gene sequencing and also for confirmatory PCR reactions for those cases in which primers derived from 5’ and 3’ gene ends failed to raise PCR products;

⁴Vector primers for 5’ and 3’ end gene sequencing.